J Hosp Infect, 1995 Jun;30(2):85-93
Autores:

Taylor, G.J.; Bannister, G.C.; Leeming, J.P.
Department of Orthopaedic Surgery, Glenfield General Hospital, Leicester, UK

Abstract:

Bacteria were counted concurrently in the air and wounds during the first 20 min of total joint arthroplasty procedures in two theatres: a conventional plenum ventilated theatre with ultraviolet C (UVC) tubes installed and a filtered vertical laminar flow theatre. Four theatre environments were tested: conventional theatre and clothing; conventional theatre with UVC protective clothing, with UVC set to produce 100 or 300 microW cm-2 s-1 irradiation; and filtered vertical laminar flow air with staff wearing cuffed cotton/polyester clothing. When used, the UVC was activated 10 min after starting an operation to assess the effect of UVC clothing alone, and of UVC radiation on bacteria already present in the wound. Compared with conventional theatres, UVC clothing reduced air counts by 38%, UVC at 100 microW cm-2 s-1 by 81%, at 300 microW cm-2 s-1 by 91%, and laminar flow by 92%. Wounds counts fell correspondingly by 66% with UVC clothing, 87% with UVC at 100 microW cm-2 s-1 and 92% both with UVC at 300 microW cm-2 s-1 and laminar flow. In conventional and laminar flow theatres air and wound counts correlated closely but in UVC theatres wound counts were lower than levels expected from prevailing air counts suggesting that UVC kills bacteria in wounds as well as in air.
Taylor, G.J.; Bannister, G.C.; Leeming, J.P.