The Laboratory Biosafety Guidelines were initially developed to guide government, industry, university, hospital, and other public health and microbiological laboratories in their development of biosafety policies and programs. The Guidelines also serve as a technical document providing information and recommendations on the esign, construction and commissioning of containment facilities.

Chapter 1
Introduction. . . . . . . . . . . . . . . . . . . . . . . 1

Chapter 2
Biological Safety . . . . . . . . . . . . . . . . . . . . 4

Chapter 3
Handling Infectious Substances. . . . . . . . . . . . 18

Chapter 4
Laboratory Design and Physical Requirements . . . 30

Chapter 5
Commissioning, Certification and Recertification
for CL3 and CL4 Laboratories. . . . . . . . . . . . . 46

Chapter 6
Large Scale Production of Microorganisms . . . . . 57

Chapter 7
Program Specific Guidelines . . . . . . . . . . . . . 63

Chapter 8
Decontamination . . . . . . . . . . . . . . . . . . . . 78

Chapter 9
Biological Safety Cabinets . . . . . . . . . . . . . . . 88

Chapter 10
Regulatory Aspects for Handling Infectious
Substances. . . . . . . . . . . . . . . . . . . . . . . . 103

Index . . . . . . . . . . . . . . . . . . . . . . . . . . . 1

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